醛固酮/盐对高血压大鼠肾脏炎症和纤维化

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所属分类:疗效
摘要

醛固酮/盐诱导大鼠炎症和纤维化高血压rats.We评价醛固酮的作用,作为肾脏炎症介质…

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醛固酮/盐对高血压大鼠肾脏炎症和纤维化

醛固酮/盐诱发的肾的炎症和纤维化的高血压大鼠

背景[123 ]

我们使用选择性醛固酮受体阻滞剂评价醛固酮的作用肾炎症和纤维化中的醛固酮/盐高血压大鼠模型中的介体,依普利酮。

的方法

[123 ] Unnephrectomized,Sprague-Dawley大鼠给予1%的NaCl(盐)喝和随机接受治疗28天:车辆输注(对照); 0.75微克/小时醛固酮皮下输注;或醛固酮输注+ 100毫克/千克/天的依普利酮口服剂量。测量血压和尿白蛋白和肾脏组织学评价。肾损伤,炎症和纤维化是由免疫组化检测,原位HYBridization,和逆转录 - 聚合酶链反应(RT-PCR)。

结果

醛固酮/盐诱发的重度高血压与对照相比(220±4毫米汞柱与131±4毫米汞柱,

,P

P < 0.05), which was partially attenuated by 依普利酮 (179 ± 4 mm Hg, 结论< 0.05). In aldosterone/salt treated rats, renal histopathologic evaluation revealed severe vascular and glomerular sclerosis, fibrinoid necrosis and thrombosis, interstitial leukocyte infiltration, and tubular damage and regeneration. Aldosterone/salt increased circulating osteopontin (925.0 ± 80.2 ng/mL vs. 53.6 ± 6.3 ng/mL) and albuminuria (75.8 ± 10.9 mg/24 hours vs. 13.2 ± 3.0 mg/24 hours) compared to controls and increased expression of proinflammatory molecules. Treatment with 依普利酮 reduced systemic osteopontin (58.3 ± 4.2 ng/mL), albuminuria (41.5 ± 7.2 mg/24 hours), and proinflammatory gene expression: osteopontin (OPN), monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6), and interleukin-1β (IL-1β).

这些结果表明,醛固酮/盐诱发的肾损伤和纤维化具有涉及巨噬细胞浸润的炎性成分和细胞因子向上-regulation通过依普利酮支持高血压性肾病醛固酮封锁的保护作用的肾损伤和炎症的衰减。

关键词

aldosteronerenalinflammation依普利酮cytokinesView摘要

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