一个哇巴因样因子是从无限增殖化细胞的下丘脑中醛固酮依赖性方式分泌

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所属分类:疗效
摘要

哇巴因样因子通过在中枢神经系统钠泵抑制和外周循环(OLF)调制血压。哇巴因…

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一个哇巴因样因子是从无限增殖化细胞的下丘脑中醛固酮依赖性方式分泌

摘要

哇巴因样因子(OLF)调制血液通过泵钠抑制压力。在中枢神经系统和外周循环。哇巴因样因子(OLF)被认为在肾上腺和下丘脑要生产,并且它可以局部地涉及到肾素 - 血管紧张素 - 醛固酮系统。然而,从体内实验使用的动物中获得后者的证据。在本研究中,我们在永生的下丘脑细胞系N1研究哇巴因生产。首先,细胞培养上清液与永生化丘脑细胞系N1为0.5,4,8和24小时收集。所用的抗哇巴因抗体新开发酶联免疫吸附测定(ELISA)表明,免疫上清液中的反应物在24显著增加与0.5小时(0.01±0.004 0.16对比±0.033皮摩尔/毫克蛋白质,,P < 0.01). A combination of HPLC and ELISA was used to characterize N1 cell-derived OLI, showing that the highest peak of OLI had the same retention time as authentic ouabain. Thereafter, N1 cells were cultured with (1–10 μM) aldosterone, and supernatant was collected after 24 h of culture. In addition, N1 cells were cultured with 5 μM 依普利酮, a mineralocorticoid receptor blocker, plus aldosterone. OLI was significantly increased in the supernatant of the cells cultured with 10 μM aldosterone (0.40 ± 0.078 pmol/mg protein), and this increase was abolished by the addition of the aldosterone antagonist 依普利酮 (0.12 ± 0.030 pmol/mg protein). These data suggest that the immortalized hypothalamic N1 cells secrete OLF and that aldosterone stimulates its secretion via mineralocorticoid receptors.

亮点

►脑哇巴因的确定在过量钠摄入血压调节的关键。►哇巴因通过永生化N1细胞,这是下丘脑来源产生。►醛固酮增强件哇巴因在培养的细胞N1释放。►哇巴因释放是通过依普利酮,盐皮质阻塞受体阻断剂。►因此,存在的由哇巴因丘脑醛固酮分泌调节。

关键词

OuabainEndogenous digitalisImmortalized丘脑cellELISAAldosteroneEplerenoneView全文

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